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Once set, plugs were dehydrated through a serial ethanol gradient and HistoChoice (Sigma) before paraffin embedding with the Histoembedder station (Leica) and cutting to 1 m sections with a Leica Jung RM2035 microtome

It helps neutralise free radicals, decrease oxidative stress, and remove harmful toxins from the body

Abstract Allicin, a bioactive compound derived from garlic, exhibits therapeutic potential against metabolic disorders but is hindered by instability and a pungent odor, limiting its clinical application

SF3B1 pull down assay coupled to mass spectrometry analysis 1 g of purified GST-tagged SF3B1(1463) in 48 l of kinase buffer (20 mM HEPES-KOH pH=7.9, 5 mM MgCl 2 , 2 mM DTT, 2 mM ATP, 1x phosphatase inhibitor cocktail (Sigma, P0044)) was incubated with 12 l flag-tagged CDK11 kinase (immunoprecipitated from HEK293 cells) or with 12 l of elution buffer (20 mM HEPES-KOH pH=7.9, 150 mM KCl, 1 mM DTT)